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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
12/06/2023 |
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Data da última atualização: |
12/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, C. P.; DORNELES, E. M. S.; HAAS, D. J.; VESCHI, J. L. A.; LOUREIRO, D.; PORTELA, R. D.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
CAROLINA PANTUZZA RAMOS, UFMG; ELAINE MARIA SELES DORNELES, UFLA; DIONEI JOAQUIM HAAS, UFMG; JOSIR LAINE APARECIDA VESCHI, CPATSA; DAN LOUREIRO, UFBA; RICARDO DIAS PORTELA, UFBA; VASCO AZEVEDO, UFMG; MARCOS BRYAN HEINEMANN, USP - São Paulo; ANDREY PEREIRA LAGE, UFMG. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Ciência Rural, v. 52, n. 11, e20210328, 2022. |
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DOI: |
http://doi.org/10.1590/0103-8478cr20210328 |
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Idioma: |
Inglês |
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Conteúdo: |
The aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis. MenosThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could ... Mostrar Tudo |
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Palavras-Chave: |
Epidemiologia molecular; ERIC 1+2-PCR; Genotipagem. |
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Thesagro: |
Doença Animal; Linfadenite Caseosa. |
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Thesaurus Nal: |
Caseous lymphadenitis; Genotyping; Molecular epidemiology. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154376/1/Molecular-characterization-of-Corynebacterium-pseudotuberculosis-2022.pdf
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Marc: |
LEADER 02705naa a2200325 a 4500
001 2154376
005 2023-06-12
008 2022 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.1590/0103-8478cr20210328$2DOI
100 1 $aRAMOS, C. P.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.$h[electronic resource]
260 $c2022
520 $aThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis.
650 $aCaseous lymphadenitis
650 $aGenotyping
650 $aMolecular epidemiology
650 $aDoença Animal
650 $aLinfadenite Caseosa
653 $aEpidemiologia molecular
653 $aERIC 1+2-PCR
653 $aGenotipagem
700 1 $aDORNELES, E. M. S.
700 1 $aHAAS, D. J.
700 1 $aVESCHI, J. L. A.
700 1 $aLOUREIRO, D.
700 1 $aPORTELA, R. D.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tCiência Rural$gv. 52, n. 11, e20210328, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/03/2007 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
CASTRO, C. S. P. de; COUTINHO, M. V.; MARQUES, A. S. dos A.; FRAZÃO, H. da S.; SANTANA, E. de F.; AMARAL, Z. P. de S. |
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Título: |
Procedimento gerencial de controle de registros. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2006. |
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Páginas: |
16 p. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Documentos, 197). |
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Idioma: |
Português |
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Palavras-Chave: |
Controle; Embrapa Recursos Genéticos e Biotecnologia; Procedimento gerencial; Registros. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CENARGEN/27982/1/doc197.pdf
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Marc: |
LEADER 00749nam a2200229 a 4500
001 1188289
005 2016-02-22
008 2006 bl uuuu u0uu1 u #d
100 1 $aCASTRO, C. S. P. de
245 $aProcedimento gerencial de controle de registros.$h[electronic resource]
260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c2006
300 $a16 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Documentos, 197).
653 $aControle
653 $aEmbrapa Recursos Genéticos e Biotecnologia
653 $aProcedimento gerencial
653 $aRegistros
700 1 $aCOUTINHO, M. V.
700 1 $aMARQUES, A. S. dos A.
700 1 $aFRAZÃO, H. da S.
700 1 $aSANTANA, E. de F.
700 1 $aAMARAL, Z. P. de S.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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